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. 2021 Jul 5;8:637122. doi: 10.3389/fmolb.2021.637122

TABLE 4.

Interaction residues of beta-sitosterol and beta-chlorogenin with Mpro complexes.

Phytocompound name H-bond Hydrophobic interaction Van der Waals interaction
Beta-sitosterol Thr24 and Thr26 Cys145 and Pro168 Thr25, Leu27, His41, Ser46, Met49, Asn142, Gly143, Ser144, His163, Met165, Glu166, Leu167, and Gln189
Beta-chlorogenin Thr25 and Cys 145 Cys145, His163, and His172 Thr24, Thr25, Ser46, Phe140, Gly143, Ser144, His164, and Met165

The bolded Lys31 and Lys353 are the important hotspot residues for S-ACE2, and His41 and Cys145 are the essential catalytic dyads for Mpro. They are actively involved in the inhibition of viral entry.