Figure 6.

Inhibition of the canonical Wnt signaling pathway by niclosamide reduces myopia development in APC^Min mice. (A) The p-β-catenin, β-catenin, Dvl3, p-GSK3β, and GSK3β levels were measured by Western blot analysis. (B) The p-β-catenin, β-catenin, Dvl3, p-GSK3β, and GSK3β were semiquantified by densitometry, normalized by β-actin levels, and expressed as relative ratios normal control group, APC^Min group, and APC^Min group treated with niclosamide. Eyes of APC^Min mice treated with niclosamide showed a significantly lower myopia (C), with a smaller VCD (D) and AL (E), compared with the wild type group and APC^Min group. Data are presented as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001. ns, no difference; 1-way ANOVA, n = 10.