Figure 4. Splicing of OAS1 exon 3 is associated with rs1131454.

a) RNA-seq plots showing splicing patterns of OAS1 exons in representative samples from nasal epithelial cells - uninfected (Mock) and Rhinovirus (RV)-infected with strains A and C. OAS1 exon 3 shows alternative splicing at both 5’ acceptor and 3’ donor splice sites, resulting in four splicing junctions – two canonical junctions (CJ) and two alternative junctions (AJ) producing Long and Short versions of exon 3. Exon junctions AJ1 (major) and AJ2 (minor) account for approximately 25% and 5% of total RNA-seq reads, respectively. Splice quantitative trait locus (sQTL) analysis of AJ1 and AJ2 with rs1131454 in b, c) nasal epithelial cells – uninfected (Mock) or infected with RVA and RVC and d, e) in PBMCs from COVID-19 patients and healthy controls (labeled as non-COVID). P-values are for linear regression adjusting for sex and age. All graphs show individual data points with error bars representing 95% confidence intervals for the medians.