Skip to main content
[Preprint]. 2021 Jul 13:2021.07.09.21260221. [Version 1] doi: 10.1101/2021.07.09.21260221

Figure 6. Nonsense-mediated decay targets OAS1 isoforms with Short exon 3 upregulated by risk rs1131454-A allele.

Figure 6.

a) RNA-seq plots showing OAS1 expression and splicing patterns in HeLa cells (rs10774671-AA, OAS1-p42) targeted by siRNA-knockdown (KD) of NMD-pathway genes SMG6 and SMG7 or by scrambled siRNA (Neg Ctrl). b) Expression of both Long and Short OAS1-p42 isoforms is increased in HeLa cells with siRNA-KD of NMD genes. c) Schematics for characterizing effects of KD of NMD genes on the expression of Long and Short exon 3 in context of OAS1-p42 (in A549) and OAS1-p46 (in HT1376). d, e) Downregulation of NMD-pathway genes (SMG6, SMG7 and UPF1) targeted by siRNA-KD in A549 cells. Expression of both Long and Short exon 3 OAS1-p42 isoforms is increased in cells with siRNA-KD of NMD genes. f, g) Downregulation of NMD-pathway genes (SMG6, SMG7 and UPF1) targeted by siRNA-KD in HT1376 cells. Only expression of Short-exon 3 OAS1-p46 isoform is increased by siRNA-KD of NMD genes. Expression was analyzed by qRT-PCR and normalized to an endogenous control (HPRT1). The dot plots present means with SD; P-values are for unpaired, two-sided Student’s t-tests.