TABLE 2.
Heat maps and coefficients of variation (CV) of the optical density (OD) values of the positive kit control for the three experiments performed in this studya
The constructed heat maps show the levels of the OD values of the positive kit control in all wells tested, from low (blue) to high (orange). Panel A shows a heat map and CV of the positive kit control of which a single dilution (1:231) was tested in 30 wells of 4 strips that were placed in the first 4 columns of a 96-well plate holder on an automated DS2 instrument (Dynex Technologies). Panel B is like panel A; however, an additional strip filled with distilled water was placed in the fifth column of the 96-well plate holder. Panel C is a heat map and CV of the positive kit control of which a single dilution (1:231) was tested in 48 wells of six strips that were placed in the first six columns of a 96-well plate holder on an automated BEP 2000 system (Siemens Healthcare Diagnostics). CV, coefficient of variation; NC, negative control; DW, distilled water.
