TABLE 1.

Sixteen samples with synonymous mutations investigated for potential N gene target failure using Xpert Xpress SARS-CoV-2

GISAID accession no.	Lineage	Clade	Ea	N2a	g.29194T>C	g.29197C>T	g.29200C>T
EPI_ISL_1575361	B.1.2	20G	25.2	27.9	x
EPI_ISL_1563712	B.1.596	20G	23.3	26.4	x
EPI_ISL_1541185	B.1.596	20G	20	22.7	x
EPI_ISL_1541162	B.1.1.519	20B	26.8	40.9		x
EPI_ISL_1541186	B.1.1	20B	28.6	NDb		x
EPI_ISL_1585891	B.1.1.519	20B	28.3	42.1		x
EPI_ISL_1541164	B.1.1.519	20B	26.7	ND		x
EPI_ISL_1541187	B.1.1	20B	30.2	43.7		x
EPI_ISL_1541165	B.1.1.519	20B	33.3	ND		x
EPI_ISL_1541166	B.1.1.519	20B	27.3	43.7		x
EPI_ISL_1541170	B.1.1.519	20B	16.1	ND		x
EPI_ISL_1541171	B.1.1.519	20B	28.4	44.3		x
EPI_ISL_1541172	B.1.1.519	20B	25.7	ND		x
EPI_ISL_1541173	B.1.1.519	20B	18.3	ND		x
EPI_ISL_1575402	B.1.2	20G	14.8	ND			x
EPI_ISL_1575358	B.1.2	20G	27	41.2			x

^aCycle threshold at which probe fluorescence was detected by PCR.

^bND, not detected.