Scheme 10. Enzymatic total synthesis of cannabinoids in a cell-free system. ThiM: hydroxyethylthiazole kinase; IPK: isopentenyl kinase; IDI: isopentyl diphosphate isomerase; GPPS: geranyl pyrophosphate synhetase; AAE3: acyl activating enzyme 3; ADK: adenylate kinase; MdcA: malonate decarboxylase α subunit; PTA: phosphotransacetylase; AckA: acetate kinase; OLS: olivetol synthase; OAC: olivetolic acid cyclase; CBGA synthase: cannabigerolic acid synthase. Starting materials are highlighted in orange. The products are highlighted in blue. Key enzymes in this synthetic route are highlighted in purple. Auxiliary enzymes are highlighted in green.