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. 2020 Jun 30;8(1):nwaa149. doi: 10.1093/nsr/nwaa149

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© The Author(s) 2020. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Transcriptome profiles of wild type and lrx345 mutant plants before and after NaCl treatment. (A) GO enrichment analysis of genes that were up-regulated in the lrx345 mutant compared with the wild type under normal conditions. The enrichment of genes responsive to eight plant hormones are shown. (B) Heat map of the 30 genes that were up-regulated with highest fold changes in the lrx345 mutant plants compared with the wild type. PDF and PR genes are indicated in red. (C) qRT-PCR analysis of the transcript levels of PDF1.2, PDF1.3, PR1 and PR5 in wild type and lrx single, double and triple mutants. ACTIN8 was used as an internal control. Values are means of three biological replicates ± SD, **P < 0.01 (Student's t-test). (D) Heat map of the abiotic stress-responsive genes that exhibited lower transcript levels in the lrx345 mutant compared to wild type after NaCl treatment for 6 h. (E, F) qRT-PCR analysis of the gene expression of RD29A and RD29B in the indicated mutant plants before and after NaCl (150 mM) (E) or ABA (50 μM) (F) treatment for 6 h. ACTIN8 was used as an internal control. Values are means of three biological replicates ± SD, **P < 0.01 (Student's t-test).