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. 2020 Jun 30;8(1):nwaa149. doi: 10.1093/nsr/nwaa149

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© The Author(s) 2020. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Disruption of the JA pathway suppresses the dwarf phenotype of the lrx345 and fer-4 mutants. (A) Rosette morphology of the wild type, lrx345, and five suppressors of lrx345 (slrx) grown in soil. Scale bar, 1 cm. (B) Petiole length of each genotype grown in soil for 4 weeks. (C) Mutations identified in the lrx suppressors by whole-genome sequencing-based mapping. (D) qRT-PCR analysis of the transcript levels of PDF1.2 and PDF1.3. ACTIN8 was used as an internal control. (E) Morphology of the upper epidermis (upper panel) and lower epidermis (bottom panel) of plants grown in soil. Scale bar, 1 cm. (F) Petiole lengths of the wild type, lrx345, coi1-1 lrx345 and aos lrx345 grown in soil for 4 weeks. (G) qRT-PCR analysis of the transcript levels of PDF1.2 and PDF1.3. ACTIN8 was used as an internal control. (H) Quantification of anthocyanin accumulation in 12-day-old seedlings of the wild type, lrx345, coi1-1 lrx345 and aos lrx345. (I) Rosette morphology of the wild type, fer-4 and coi1-1 fer-4. Scale bar, 1 cm. (J) Petiole lengths of the wild type, fer-4 and coi1-1 fer-4 grown in soil for 4 weeks. (K) qRT-PCR analysis of the transcript levels of PDF1.2 and PDF1.3 in wild type, fer-4 and coi1-1 fer-4. ACTIN8 was used as an internal control. Values in (B), (F) and (J) are means of 10 petioles ± SD. Values in (D), (G), (H) and (K) are means of three biological replicates ± SD. Different letters represent statistically significant differences (P < 0.01, one-way ANOVA).