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. 2020 Jun 30;8(1):nwaa149. doi: 10.1093/nsr/nwaa149

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© The Author(s) 2020. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Suppression of the salt-hypersensitive phenotype of lrx345 mutant plants by a mutation in ABA2. (A) Phenotypes of seedlings of the indicated genotypes grown on MS and MS+NaCl (120 mM) media. (B) Survival rates of the wild type, lrx345 and aba2-1 lrx345 seedlings grown on MS+NaCl (120 mM) medium. (C) Phenotypes of seedlings transferred from MS to MS+NaCl (100 mM) medium. The photograph was taken 5 days after 3-day-old seedlings were transferred. (D) Survival rate of the wild type, lrx345 and aba2-1 lrx345 seedlings grown on MS+NaCl (120 mM) media supplemented with or without ABA (1 μM). (E) ABA contents of 10-day-old seedlings before and after NaCl (150 mM) treatment (1 h). (F) qRT-PCR analysis of the transcript levels of PDF1.2, PDF1.3, PR1 and PR5 in the indicated genotypes. ACTIN8 was used as an internal control. Values in (B–F) are means of three biological replicates ± SD, *P < 0.05 and **P < 0.01 (Student's t-test). (G) Measurement of JA and SA contents in the wild type, lrx345 and aba2-1 lrx345 seedlings. Values are means of three biological replicates ± SD. Different letters represent statistically significant differences (P < 0.01, one-way ANOVA). (H) qRT-PCR analysis of the expression of RD29A and RD29B in the indicated genotypes before and after NaCl treatment. ACTIN8 was used as an internal control. Values are means of three biological replicates ± SD.