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. 2020 Jun 30;8(1):nwaa149. doi: 10.1093/nsr/nwaa149

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© The Author(s) 2020. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Inhibition of ROS production restores the cell-death phenotype of lrx345 and fer-4 mutants under high salinity. (A) Transcript levels of RbohD, RbohF and six oxidative stress-responsive genes before and after NaCl treatment. The expression values of each gene were calculated based on RNA-seq data. (B) Wild type, lrx345 and fer-4 seedlings before and after NaCl treatment were stained with DAB and NBT. (C) Phenotype of seedlings grown on MS+NaCl (120 mM) media supplemented with or without DMTU (5 mM). (D) Survival rate of the wild type, lrx345 and fer-4 seedlings grown on MS+NaCl (120 mM) media supplemented with or without DMTU (5 mM). Values are means of three biological replicates ± SD, **P < 0.01 (Student's t-test). (E) Diagram summarizing the role of LRX3/4/5-RALF22/23-FER module in the regulation of JA, SA, ABA and ROS accumulation.