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Key factors of dephasing patterns revealed by single-cycle simulation. (a) Dephasing parameters and their corresponding nascent DNA strands. (b) Insufficient Pol will cause severe lag, which can be remedied by prolonged reaction time. (c) Distribution of ϵ1 percentage in AGGAAA and ϵ2 percentage in AGAAA of each set of parameters, which gives the definition of the OBS index.
