Table 1.
Adult hematopoietic cell quantitations
| WT | gata2b−/− | |
|---|---|---|
| Scatter analysis in frequency of single viable cells | n = 26 | n = 23 |
| Erythrocytes in KM (3-12 mo) | 25.4 ± 2.8 | 25.9 ± 3.0 |
| Progenitors in KM (3-12 mo) | 26.8 ± 2.0 | 24.4 ± 1.9 |
| Lymphocytes and HSCs in KM (3-12 mo) | 18.1 ± 1.1 | 29.0 ± 1.9**** |
| Myeloid cells in KM (3-12 mo) | 20.3 ± 1.4 | 10.3 ± 0.9**** |
| Reporter expression in frequency of single viable cells | ||
| Tg(mpx:GFP) (GFP+ in single live cells) | 17.2 ± 3.0 (n = 8) | 5.1 ± 1.0 (n = 7)** |
| Tg(mpeg:GFP)gl22Tg (GFP+ in single live cells) | 3.5 ± 1.9 (n = 4) | 2.1 ± 0.7(n = 6) |
| Tg(LCK:GFP) (GFP+ in single live cells) | 3.1 ± 0.8 (n = 4) | 3.4 ± 0.6 (n = 4) |
| Tg(CD41:GFP) (GFP low in single live cells) | 0.4 ± 0.1 (n = 9) | 0.4 ± 0.1 (n = 8) |
Data are given as mean ± standard error of the mean. n indicates number of zebrafish used in analysis.
**P < .01, ****P < .0001. If data are normally distributed, we used 1-way analysis of variance with Tukey posttest. If data are not normally distributed, we used Kruskal-Wallis with Dunn posttest.