Figure 1.

Study design. (a) Overview of the viral antigens included in our study and evaluated for performance in antibody detection. (b) Overview of the antigen evaluation. In the first stage, antigens providing antibody detection in a majority of a small set of positive samples were selected. This was followed by the second stage where 12 selected antigens were evaluated for their sensitivity and specificity. In an intermediate set of eight antigens, we also investigated the influence of different protein production systems in relation to the outcome of the analysis. In the final stage, five antigens were further assessed for sensitivity and specificity in the full sample set, both separate and combined in panels.