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. 2021 Jul 7;19(7):e3001334. doi: 10.1371/journal.pbio.3001334

Fig 6. glp-1 and lin-12 Notch receptors and sel-8 coactivator are not required for ADF specification.

Fig 6

(A) Schematic representation of Notch signalling pathway in C. elegans. (B) P0 RNAi and double mutant analysis for glp-1 and lin-12 Notch receptors and temperature-sensitive allele analysis for glp-1 show no significant difference in tph-1(otIs517) and cat-1(otIs221) reporter expression in the ADF neuron. L1 stage confocal images showing representative examples of the temperature shift assay. Scale bar: 15 μm. See S1 Data for numerical values of all replicates and temperature-sensitive allele scoring at YA stage. (C) P0 RNAi and mutant analysis of sel-8 coactivator shows no difference in tph-1(otIs517) and cat-1(otIs221) reporter expression in the ADF neuron. L1 stage confocal images showing representative examples. See S1 Data for numerical values. L1, first larval stage; NICD, Notch intracellular domain; n.s, nonsignificant; YA, young adult.