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. 2021 Jul 7;19(7):e3001334. doi: 10.1371/journal.pbio.3001334

Fig 7. LAG-1 mediates tph-1 expression induction by specific stimuli.

Fig 7

(A) ADF mNeonGreen intensity quantification of tph-1(vlc46) endogenous reporter in dauer stage shows a significant increase compared to L4. n > 60 neurons. Median and IQR are represented. See S1 Data for numerical values. (B) Dauer animals show increased ADF yzIs71(tph-1::gfp) reporter expression both in lag-1 RNAi and control animals, suggesting that LAG-1 does not mediate tph-1 induction in dauer. Median and IQR are represented. See S1 Data for numerical values. (C) L1 ADF mNeonGreen intensity quantification of tph-1(vlc46) upon heat shock. Heat shock induces tph-1 expression both in wild-type and lag-1(om13) mutant worms, suggesting that lag-1 is not required in the heat response. Median and IQR are represented. See S1 Data for numerical values. (D) L1 ADF mNeonGreen intensity quantification of tph-1(vlc46) endogenous reporter in unc-43(n498)/CAMKII gain of function mutation. unc-43(n498gof) induces tph-1 expression both in wild-type and lag-1(om-13) mutants, suggesting that lag-1 is not required to mediate response to neuronal activation. Median and IQR are represented. See S1 Data for numerical values. (E) L1 ADF mNeonGreen intensity quantification of tph-1(vlc46) endogenous reporter in dyf-1(yz66) mutants shows increased levels of tph-1 expression that are not observed in dyf-1(yz66); lag-1(om13) double mutants, suggesting that lag-1 mediates tph-1 expression response to cilia damage. Median and IQR are represented. See S1 Data for numerical values. (F) Similar lag-1 dependency of tph-1(vlc46) endogenous reporter induction is observed in an additional intraflagellar transport protein mutant, che-11(e1810). Median and IQR are represented. See S1 Data for numerical values. (G) Young adult ADF mNeonGreen intensity quantification of tph-1(vlc46) endogenous reporter in tir-1(yz68gof) gain-of-function mutation, which mimics response to pathogenic bacteria. Median and IQR are represented. See S1 Data for numerical values. (H) tir-1(yz68gof) induction of yzIs71(tph-1::gfp) reporter is not observed in animals treated with lag-1 RNAi, suggesting that lag-1 mediates tph-1 expression response to pathogenic bacteria. Median and IQR are represented. See S1 Data for numerical values. IQR, interquartile range; n.s., nonsignificant; YA, young adult.