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. 2021 Jul 19;16(7):e0254815. doi: 10.1371/journal.pone.0254815

Fig 1. Schematic of the CRISPR-Cas12a based assay for ASFV detection.

Fig 1

Steps are summarized as follows: (1) Extraction of the DNA from pig tissues; (2) Amplification of the target using RPA method; (3) Cas12a cleavage; (4) Fluorescence signals detected by the naked eye.