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(A). Schematic of the p72 gene, and the corresponding locations of each crRNA PAM region. (B-F). Fluorescence signals detected by five crRNAs targeting the ASFV p72 gene designed for the detection of ASFV. Background fluorescence by the individual crRNA in the absence of target DNA was shown as “CT”. n = three technical replicates, bars represent mean ± SD, ***p <0.001.
