Fig 4. Detection of ASFV using RPA coupled with Cas12a-based assay.

(A). Visualization of RPA products by agarose gel electrophoresis. The expected product size is 300 bp. (B). Reactions were performed after an RPA amplification of the virus DNA with varying initial concentrations, and it can detect as low as two copies of initial DNA. n = three technical replicates, bars represent mean ± SD, ***p <0.001. (C). Direct observation by the naked eye when the Cas12a reactions system is exposed to blue light. CT, negative control.