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. 2021 Feb 4;296:100376. doi: 10.1016/j.jbc.2021.100376

Figure 3.

Figure 3

Knockdown of lnc-ORA inhibits myoblast proliferation.A, EdU and DAPI (nuclei) staining analysis 24 h after transfection of silnc-ORA and si-NC in proliferating myoblasts (n = 3). The scale bar represents 200 μm. B, percentage of EdU-positive cells/total cells (n = 3). C, flow cytometry analysis 24 h after transfection of silnc-ORA and si-NC in proliferating myoblasts (n = 3). D, statistical results of flow cytometry (n = 3). E, mRNA levels of cyclin E, cyclin D1, and PCNA 24 h after knockdown of lnc-ORA (n = 3). F, Western blot detection of cyclin E, cyclin D1, and PCNA (n = 3). G, quantitation of protein level in F (n = 3). Data represent the mean ± SD. ∗p < 0.05; ∗∗p < 0.01. DAPI, 4′,6-diamidino-2-phenylindole; EdU, 5-ethynyl-20-deoxyuridine; lnc, long noncoding; lnc-ORA, obesity-related lncRNA; PCNA, proliferating cell nuclear antigen; silnc-ORA, siRNA-lnc-ORA.