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. 2021 Feb 4;296:100376. doi: 10.1016/j.jbc.2021.100376

Figure 4.

Figure 4

Overexpression of lnc-ORA inhibits myogenic differentiation.A, immunofluorescent staining of MyHC and DAPI (nuclei) on day 4 of differentiation after overexpression of lnc-ORA (n = 3). The scale bar represents 100 μm. B, statistical analysis of the differentiation index in A (n = 3). C, statistical analysis of the fusion index in A (n = 3). D, mRNA levels of myogenic genes on day 4 of differentiation after overexpression of lnc-ORA (n = 3). E, Western blot detection of MyoD, MyoG, and MyHC (n = 3). F, quantitation of protein levels in E (n = 3). Data represent the mean ± SD. ∗p < 0.05; ∗∗p < 0.01. DAPI, 4′,6-diamidino-2-phenylindole; lnc, long noncoding; lnc-ORA, obesity-related lncRNA; MyHC, myosin heavy chain; MyoD, myogenic differentiation 1; MyoG, myogenin.