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. 2021 Feb 4;296:100376. doi: 10.1016/j.jbc.2021.100376

Figure 7.

Figure 7

Overexpression of lnc-ORA decreases muscle mass and induces muscle atrophy in vivo.A, images of mouse skeletal muscles. B, statistical analysis of muscles mass (n = 5). C, percentage of skeletal muscle (TA and GAS) (n = 5). D, H&E staining images of GAS cross section. The scale bar represents 200 μm. E, statistics of fiber area in D (n = 5). F, Western blot detection of MyHC, MyoD, MAFbx, MuRF, p-PI3K, PI3K, p-AKT, and AKT in the GAS (n = 3). G and H, quantitation of protein level in F (n = 3). Data represent the mean ± SD. ∗p < 0.05; ∗∗p < 0.01. AKT, protein kinase B; GAS, gastrocnemius; lnc, long noncoding; lnc-ORA, obesity-related lncRNA; MAFbx, muscle atrophy F-box; MuRF, Muscle RING finger; MyHC, myosin heavy chain; MyoD, myogenic differentiation 1; p-AKT, phosphorylated AKT; p-PI3K, phosphorylated PI3K; TA, tibialis anterior.