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. 2021 Feb 4;296:100376. doi: 10.1016/j.jbc.2021.100376

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Subcellular localization of lnc-ORA in proliferating and differentiating myoblasts.A, subcellular localization of lnc-ORA by FISH in myoblasts. Special FISH probes against lnc-ORA, U6, and 18S were modified by Cy3 in red. 18S is a cytoplasmic marker, and U6 is a nuclear marker. The nucleus was stained by DAPI in blue. The scale bar represents 50 μm. B, subcellular localization of lnc-ORA by FISH in myotubes. Special FISH probes against lnc-ORA, U6, and 18S were modified by Cy3 in red. 18S is a cytoplasmic marker, and U6 is a nuclear marker. The nucleus was stained by DAPI in blue. The scale bar represents 50 μm. DAPI, 4′,6-diamidino-2-phenylindole; lnc, long noncoding; lnc-ORA, obesity-related lncRNA.