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. 2021 Feb 4;296:100376. doi: 10.1016/j.jbc.2021.100376

Figure 9.

Figure 9

Lnc-ORA functions as a ceRNA for miR-532-3p to control myogenesis.A and B, schematic of the double luciferase assay vector. C and D, analysis of the luciferase reporter assay (n = 3). E, EdU and DAPI (nuclei) staining analysis. The scale bar represents 200 μm. F, statistical analysis in E (n = 3). G, immunofluorescent staining of MyHC analysis. The scale bar represents 200 μm. H, statistical analysis in G (n = 3). I, Western blot detection of PTEN and myogenic factors (n = 3). J, statistical analysis in I (n = 3). Data represent the mean ± SD. ∗p < 0.05; ∗∗p < 0.01. ceRNA, competing endogenous RNA; DAPI, 4′,6-diamidino-2-phenylindole; EdU, 5-ethynyl-20-deoxyuridine; lnc, long noncoding; lnc-ORA, obesity-related lncRNA; MyHC, myosin heavy chain; PTEN, phosphatase and tensin homolog.