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. 2020 Jan 12;7(4):815–822. doi: 10.1093/nsr/nwz206

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© The Author(s) 2020. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Isolated chondrocytes of Hypacrosaurus and their positive response to two DNA assays. (A, B, E) Isolated chondrocytes of Hypacrosaurus and emu photographed under transmitted light (green arrows). Hypacrosaurus chondrocytes were successfully isolated as individual cells (A) and cell doublets (B). Hypacrosaurus (C) and emu chondrocytes (F) showing positive response to propidium iodide (PI), a DNA intercalating dye, to a small and circular region that locates intracellularly (white arrows). Hypacrosaurus (D) and emu chondrocytes (G) also show a similar binding when exposed to 4^′,6^′-diamidino-2-phenylindole dihydrochloride (DAPI), another DNA-specific stain (black arrows) although in both cases, emu cell staining is significantly greater than in the dinosaur cells.