Table 2.
Polymer-degrading potential of the soda soil actinomycete strain ACPA22T in growth experiments (G), activity tests with dye-conjugated polymers (A) and encoded glucosidases in the genome (GH)
|
Polymer |
G |
A |
Genomic |
|
|---|---|---|---|---|
|
GH family |
Function |
|||
|
α-Glucans | ||||
|
Amylose, dextrin, cyclodextrin, pullulan glycogen |
+ |
+ |
2 × GH15 |
Trehalase |
|
GH13_3 |
Pullulanase |
|||
|
GH13_32 |
α-Amylase |
|||
|
Dextran ( Leuconostoc ) |
− |
− |
GH49 (−) |
Dextranase |
|
β-Glucans | ||||
|
Carboxymethyl-cellulose |
− |
Weak* |
GH16_3 |
β-1,3(4)(6)-Endoglucanases |
|
Amorphous cellulose |
− |
nd |
||
|
Barley glucan |
− |
− |
||
|
Laminarin |
+ |
nd |
||
|
Lichenan |
+ |
− |
||
|
Pachyman/curdlan |
− |
− |
GH81 (−) |
β-1,3-Endoglucanases |
|
Xylan |
− |
− |
GH10 (−) |
β-1,4-Endoxylanase |
|
Amorphous chitin |
− |
nd |
GH18/19/20 |
Chitinases |
|
Other glucans including heteropolysaccharides | ||||
|
Alginate |
− |
nd |
PL (−) |
Alginate lyase |
|
Rhamnogalacturonan |
nd |
Weak |
GH88/105 (−) |
Rhamnogalacturonyl hydrolase |
|
β-Mannan |
− |
− |
GH5/22/26 (−) |
β-Mannanase |
|
Arabinan |
− |
− |
GH43 |
Arabinase |
|
Galactan |
− |
− |
GH54 (−) |
β-1,4-Endogalactanase |
|
Glucomannan |
Weak |
− |
|
|
|
Xyloglucan |
− |
− |
GH5/GH9 (−) |
Endoxyloglucanase |
|
Arabinoxylan |
− |
− |
GH10/GH43 (−) |
Arabinase/xylanase |
|
Pectin |
− |
− |
PL1 (−) |
Pectate lyase |
|
Inulin |
+ |
nd |
GH32 (−) |
Inulinase |
|
Proteins | ||||
|
Casein |
+ |
+ |
nd |
|
|
Gelatin |
+ |
+ |
nd |
|
nd, Not determined.
*Tested in plate assay with Congo red flooding.
**Negative in the dye-conjugated assay, but positive in plate assay with Lugol solution flooding.
(-), indicate absence of the corresponding GH genes; shading, Indicate positive results.