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Impact of substrate mechanics on MSCgene and secretome expression. (A) Hierarchical cluster analysis of gene expression of MSCs cultured on silk hydrogels for 14 days using pooled RNA isolation from four MSC donors. Differential gene expression was calculated and shown as log2 fold change. (B) Expression pattern of secreted proteins over 14 days. A conditioned culture medium was pooled from four MSC donors prior to analysis.
