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. 2021 Apr 22;18(5):1895–1904. doi: 10.1021/acs.molpharmaceut.0c00574

Table 3. Primary and Secondary Antibodies Used for the Analysis of P-gp Protein Content in Human and Rat Intestines by Western Blot.

Protein Primary antibody (dilution ratio; supplier) Secondary antibody (dilution ratio; supplier)
Human
P-gp Human monoclonal anti-P-gp C-494 (3:200; Enzo Life Science, Exeter, U.K.) Affinity-purified rabbit anti-human IgG coupled to horseradish peroxidase (1:5000; Sigma-Aldrich, Poole, U.K.)
β-actin Anti-β-actin human monoclonal antibody (1:2000, ThermoFisher, Loughborough, U.K.)  
Rat
P-gp Mouse monoclonal anti-P-gp C-219 (3:200; Enzo Life Science, Exeter, U.K.) Affinity-purified rabbit anti-mouse IgG coupled to horseradish peroxidase (1:5000; Sigma-Aldrich, Poole, U.K.)
β-actin Anti-β-actin mouse monoclonal antibody (1:2000; Sigma-Aldrich, Poole, U.K.)