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. 2021 Jun 30;36(8):2249–2274. doi: 10.1093/humrep/deab123

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© The Author(s) 2021. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Characterization of UF-EVs. (A) Particle size distributions by NTA in proliferative (pUF-EVs; n = 5) and secretory phase samples (sUF-EVs; n = 5) (left panel). Data are displayed as dot-plots showing mean and SD. Particle concentrations/ml by NTA in pUF-EVs and sUF-EV samples (right panel). Data are displayed as boxplots from the minimum to the maximum concentration value, showing median (line) concentration value. Underneath the graph, descriptive table reporting size distribution of UF-EVs collected in proliferative (pUF-EVs) and secretory (sUF-EVs) phase of the cycle. Data represent the median value of mode, D10 (10th percentile), D50 (50th percentile) and D90 (90th percentile) with interquartile range (IQR). (B) Transmission electron microscopy (TEM) representative illustrations of pUF-EVs (left image) and sUF-EV samples (right image) with 100–200 nm size. Scale bar column: 200 nm. The inset represents an area with clear extracellular vesicles structures (C) Western blot showing the presence of different canonic EV markers (CD63, CD9, ALIX, TSG101) in both pUF-EVs and sUF-EV. Two different samples/phase were included. NTA, nanoparticle tracking analysis; UF-EVs, uterine fluid-derived extracellular vesicles.