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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: FASEB J. 2020 Oct 13;34(12):16034–16048. doi: 10.1096/fj.202001192R

Figure 1. Chronic exposure to low dose iAs promotes clonogenicity, invasiveness and the proliferation of luminal epithelial breast cancer cells.

Figure 1.

(A) Matrigel™ invasion assay using parental MCF-7 cells (control) and MCF-7 cells cultured in the presence of iAs (100 nM) for 300 days (iAs-300). Cells were platted on the upper chamber of a transwell setup on top of a thin layer of Matrigel™ and allowed to migrate towards the bottom chamber filled with complete media. Cells were stained and counted after 18h. (B) Organoid formation assay using cells platted in Matrigel™ 5% and grown for up to 30 days. Organoids were counted using an inverted microscope. (C) Proliferation curves of MCF-7 and MCF7-iAs300 co-stimulated with E2 (10 nM) for the indicated time points. All experiments represent averages ±SEM of 3 independent biologic replicates. * p < 0.05; *** p<0.001.