Figure 3. Juniperus communis (JCo) extract induces G0/G1 arrest and regulates the expression of cell cycle-associated proteins in HT-29 cells. A, HT-29 cells were treated with 65 μg/mL JCo extract for 0, 6, 12, 24, and 48 h, stained with propidium iodide, and analyzed for FL2 intensity by flow cytometry. B, Cell cycle distribution (G0/G1, S, and G2/M phases) in JCo extract-treated cells was analyzed using Kaluza Flow Cytometry Analysis software. Data are reported as means±SD. *P<0.05 vs control with a significant increase, #P<0.05 vs control with a significant decrease (ANOVA). C, The expression of cell cycle-associated proteins in JCo extract-treated cells was determined by western blotting.
