Figure 4. A and B, human umbilical vein endothelial cell (HUVEC) migration after treatment for 24 h with engeletin (50, 100, 200, 400 nM) was assessed via Transwell or wound healing assay. HUVEC tube formation was assessed via tube formation assay. C, Effects of engeletin and axitinib (1 μM) on HUVEC tube formation (scale bar 100 μm). D, Transwell assay migration cell count. E, Relative migration in the wound healing assay. F, Number of tubes in the tube formation assay. *P<0.05, **P<0.01 vs control group (ANOVA with Tukey's test).
