Table 3.

Top six ASVs (377-378 bp long) of 18S rRNA genes aligned and assigned to Chlorophyta in the samples, the closest relatives of the 18S rRNA genes that are based on the Nucleotide BLAST search in NCBI, and the sequence identity of 18S rRNA gene (%).

ASV No.	Relative abundance (%)	Closest identified relatives	Accession numbers	Sequence identity (%)
ASV 1	45.6	Sanguina nivaloides CCCryo RS 0015-2010	JQ790560.1	100
		Sanguina aurantia CCCryo RS 0017-2010	MK728645.1	100
ASV 2	32.1	Chloromonas hindakii WP129/CCCryo 531-19	MN251865.1	100
		Chloromonas polyptera DRAnt023	JQ790556.1	100
		Chloromonas sp. Gassan-B	LC012714.1	100
		Chloromonas sp. TA-8	AB902996.1	100
ASV 3	7.6	Uncultured alga: OTU003	LC371421.1	100
		Chloromonas sp. TA-9	AB903024.1	100
		Chloromonas sp. SV-3	AB903022.1	100
		Chloromonas_sp_PA2	AB903020.1	100
		Chloromonas_sp_GL4	AB902991.1	100
		Chloromonas_sp_JP21	AB903006.1	100
		And many others
ASV 4	3.6	Uncultured alga: Otu025	LC371427.1	99.2
		Uncultured alga: Otu005	LC371425.1	99.2
		Uncultured alga: Otu003	LC371421.1	99.2
		Chloromonas sp. TA-9	AB903024.1	99.2
		Chloromonas sp. SV-3	AB903022.1	99.2
		And many others
ASV 5	3.5	Chloromonas_sp_TA_1	AB903004.1	100
		Chloromonas_sp_TA_3	AB902981.1	100
		Uncultured alga: OTU008	LC371432.1	99.5
		Chlainomonas sp. LP03	MF803745.1	98.7
		Chlainomonas sp. DL06	MF803743.1	98.7
ASV 6	2.5	Chloromonas hindakii WP129/CCCryo 531-19	MN251865.1	99.7
		Chloromonas polyptera DRAnt023	JQ790556.1	99.7
		Chloromonas sp. Gassan-B	LC012714.1	99.7
		Chloromonas sp. TA-8	AB902996.1	99.7

Top six ASVs were chosen which contained more than 1% in total ASVs.