Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain (Mus musculus), C57BL/6JOla, male and female | Nfasc-/- mice | Sherman et al., 2005 | Peter Brophy, University of Edinburgh | |
| Strain (Mus musculus), C57BL/6JOla, male and female | Cntnap1-/- mice | Gollan et al., 2003 | E Peles, Weitzmann Institute |
|
| Strain (Mus musculus), C57BL/6JOla, male and female | Thy1-SEP-Nfasc186 | This paper | Peter Brophy, University of Edinburgh | |
| Strain (Mus musculus), C57BL/6JOla, male and female | ß1Nav-EGFP | Booker et al., 2020 | Peter Brophy, University of Edinburgh | |
| Antibody | P0 (chicken polyclonal) |
Aves Labs | RRID:AB-2313561 | 1:100 |
| Antibody | Sodium channel (Pan) (mouse monoclonal) | Sigma-Aldrich | Cat# S8809 | 1:200 |
| Antibody | MAG (mouse monoclonal) | M Filbin | IgG1 | 1:50 |
| Antibody | ßIV-spectrin (rabbit polyclonal) |
Desmazieres et al., 2014 | PJ Brophy | 1:200 |
| Antibody | ßIV-spectrin (rabbit polyclonal) |
MN Rasband, Baylor College of Medicine | 1:400 | |
| Antibody | AnkyrinG (mouse monoclonal) | UC Davis/NIH NeuroMab | Cat# 75–147 | 1:50 |
| Antibody | GFP (chicken polyclonal) |
Abcam | Cat# ab13970 | 1:1000 |
| Antibody | β3-tubulin (mouse monoclonal) |
Sigma-Aldrich | Cat# T8660 | 1:100 |
| Antibody | Gliomedin (rabbit polyclonal) |
E Peles | 1:100 | |
| Antibody | Caspr (rabbit polyclonal) |
DR Colman | 1:5000 | |
| Antibody | Caspr (guinea-pig polyclonal) |
M Bhat, University of Texas, San Antonio | 1:400 | |
| Antibody | MBP (rabbit polyclonal) |
Vouyioukiis and Brophy, 1993 | PJ Brophy, pep7 | 1:1000 |
| Antibody | Periaxin (rabbit polyclonal) |
Gillespie et al., 1994 | PJ Brophy, Repeat region |
1:5000 |
| Antibody | pERM (rabbit polyclonal) |
Cell Signalling Technologies | Cat# 3141 | 1:200 |
| Antibody | Dystrophin (mouse monoclonal) |
Sigma-Aldrich (MANDRA1) | Cat# D8043 | 1:200 |
| Antibody | Radixin (rabbit polyclonal) |
Sherman et al., 2012 | P Brophy, RAD4 | 1:500 |
| Antibody | Neurofascin-Pan (rabbit polyclonal) | Tait et al., 2000 | PJ Brophy, NFC | 1:2000 |
| Antibody | Goat Anti-mouse IgG1 Alexa Fluor 488 |
Invitrogen | Cat# A-21121 | 1:1000 |
| Antibody | Goat Anti-rabbit Alexa Fluor 647 |
Invitrogen | Cat# A-32733 | 1:1000 |
| Antibody | Donkey Anti-rabbit Alexa Fluor 594 |
Jackson ImmunoResearch | Cat# 111-585-14 | 1:1000 |
| Antibody | Donkey Anti-chicken Alexa Fluor 488 |
Jackson ImmunoResearch | Cat# 703-545-155 | 1:1000 |
| Antibody | Goat Anti-mouse IgG2a Alexa Fluor 568 | Invitrogen | Cat# A-21134 | 1:1000 |
| Antibody | Goat Anti-mouse IgG2a Alexa Fluor 647 | Invitrogen | Cat# A-21241 | 1:1000 |
| Antibody | Goat Anti-mouse IgG2b Alexa Fluor 488 | Invitrogen | Cat# A-21141 | 1:1000 |
| Antibody | Anti-mouse IgG1 Alexa Fluor 594 | Invitrogen | Cat# A-21125 | 1:1000 |
| Antibody | Goat Anti-mouse IgG1 Alexa Fluor 647 | Invitrogen | Cat# A-21240 | 1:1000 |
| Antibody | Goat Anti-mouse IgG2b DyLight 405 | Jackson ImmunoResearch | Cat# 115-475-207 | 1:100 |
| Antibody | Goat Anti-guinea pig Alexa Fluor 594 |
Invitrogen | Cat# A-11076 | 1:8000 |
| Antibody | Goat Anti-rabbit Alexa Fluor 488 |
Invitrogen | Cat# 710369 | 1:1000 |
| Antibody | Goat Anti-chicken Alexa-Fluor 647 |
Invitrogen | Cat# A-21449 | 1:1000 |
| Chemical compound, drug | Phalloidin AlexaFluor 568 | Invitrogen | Cat# A-12380 | 1:50 |
| Chemical compound, drug | DAPI (4 ′, 6-diamidino-2-phenylindole) | Sigma-Aldrich | Cat# D9542 | 1 μg/ml |
| Chemical compound, drug | BrainStain fluorescent dye mix | ThermoFisher | Cat# B34650 | 1:300 |
| Chemical compound, drug | DMSO | Sigma-Aldrich | Cat# D2650 | |
| Chemical compound, drug | Poly-D-lysine | Sigma-Aldrich | Cat# P6407 | |
| Chemical compound, drug | B-27 | Thermo Fisher Scientific | Cat# 17504044 | |
| Chemical compound, drug | Matrigel | Corning | Cat# 356231 | |
| Chemical compound, drug | Fish skin gelatin | Sigma-Aldrich | Cat# G7765 | |
| Chemical compound, drug | Nocodazole | Sigma-Aldrich | Cat# SML1665 | |
| Chemical compound, drug | Latrunculin A | Merck | Cat# 428026 | |
| Chemical compound, drug | para-amino-Blebbistatin | Cayman Chemical | Cat# 22699 | |
| Software, algorithm | FIJI | Schindelin et al., 2012 | RRID:SCR_002285 | https://imagej.net/Fiji |
| Software, algorithm | Prism 8.0 | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | KymoTool Box | Zala et al., 2013 | F Saudou, University Grenoble Alpes |
|
| Software, algorithm | Amira | ThermoFisher | RRID:SCR_007353 |