Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) |
Robo1tm1Matl
;Robo2tm1Rilm |
PMID:26743624 | RRID:MGI:3043177
RRID:MGI:5705332 |
Linked alleles provided by Xin Sun |
| Genetic reagent (M. musculus) | Ins1tm1.1(cre)Thor | Jackson Laboratory | RRID:IMSR_JAX:026801 | |
| Genetic reagent (M. musculus) | B6.FVB(Cg)-Tg(Ucn3-cre)KF43Gsat/Mmucd | MMRRC | RRID:MMRRC_037417-UCD | |
| Genetic reagent (M. musculus) | R26H2BCherry | PMID:25233132 | R26H2BCherry | Generated as described in source |
| Genetic reagent (AAV8) | VB171020-1118pud | PMID:31186447
Generated by VectorBuilder |
AAV8-INS-GcAMP6s | Sequence available in Supplementary materials of source |
| Antibody | Anti-insulin (guinea pig polyclonal) | Agilent | Cat#:IR002 RRID:AB_2800361 | (1:6) |
| Antibody | Anti-glucagon (mouse monoclonal) | Sigma | Cat#:G2654 RRID:AB_259852 | (1:500) |
| Antibody | Anti-glucagon (rabbit polyclonal) | Cell Signaling | Cat#:2760S RRID:AB_659831 | (1:200) |
| Antibody | Anti-somatostatin (rabbit polyclonal) | Phoenix | Cat#:G-060-03 RRID:AB_2890901 | (1:1000) |
| Antibody | Anti-Cx36 (rabbit polyclonal) | Invitrogen | Cat#:36-4600 RRID:AB_2314259 | (1:80) |
| Antibody | Anti-Col IV (rabbit polyclonal) | Abcam | Cat#:Ab6586 RRID:AB_305584 | (1:300) |
| Antibody | Anti-laminin β1 (rat monoclonal) | Invitrogen | Cat#:MA5-14657 RRID:AB_10981503 | (1:500) |
| Antibody | Anti-Tubb3 (rabbit polyclonal) | BioLegend | Cat#:poly18020 RRID:AB_2564645 | (1:4000) |
| Antibody | Anti-guinea pig 594 (donkey polyclonal) | Jackson | Cat#:706-585-148 RRID:AB_2340474 | (1:500) |
| Antibody | Anti-guinea pig 647 (donkey polyclonal) | Jackson | Cat#:706-605-148 RRID:AB_2340476 | (1:500) |
| Antibody | Anti-rabbit 594 (donkey polyclonal) | Invitrogen | Cat#:A21207 RRID:AB_141637 | (1:500) |
| Antibody | Anti-goat 647 (donkey polyclonal) | Invitrogen | Cat#:A21447 RRID:AB_141844 | (1:500) |
| Antibody | Anti-rat 488 (donkey polyclonal) | Invitrogen | Cat#:A21208 RRID:AB_141709 | 1:500 |
| Antibody | Anti-rabbit 488 (donkey polyclonal) | Invitrogen | Cat#:A21206 RRID:AB_2535792 | (1:500) |
| Antibody | Anti-mouse 488 (donkey polyclonal) | Jackson | Cat#:715-546-151 RRID:AB_2340850 | (1:500) |
| Antibody | Anti-biotin 488 (mouse polyclonal) | Jackson | Cat#:200-542-211 RRID:AB_2339040 | (1:500) |
| Chemical compound, drug | Phalloidin-647 | Invitrogen | A22247 | (1:400) |
| Chemical compound, drug | 2-NBDG | Invitrogen | N13195 | (5 mg/mL) |
| Chemical compound, drug | Fura2-AM | Thermo Fischer | F1201 | (5 μm) |
| Peptide, recombinant protein | Accutase | Thermo Fischer | A1110501 | |
| Peptide, recombinant protein | Geltrex | Thermo Fischer | A1413302 | |
| Software, algorithm | Prism | GraphPad | RRID:SCR_005375 | |
| Software, algorithm | MATLAB | MathWorks | RRID:SCR_001622 |