Table 1. Embalming procedure performed at University of Florida for Prof. Rhoton's Laboratory.

Steps	Description
1	Section and cannulation of the carotid artery (its superior part clamped)
2	Two perfusions toward the feet with 2 L of 37% formaldehyde + 10 L of water. The jugular vein should be divided and left open to drain the solution a
3	One perfusion with 3 L of solution A (40% phenol, 40% alcohol, 17% formaldehyde at 37%, 3% maquat 50—antifungal) + 9 L of water + 200 mL of glycerin a
4	Separate the specimen (head) from the body.
5	Perfusion through both carotid arteries (one at time), with 1 L of solution A + 11 L of water. Vertebral arteries and the contralateral carotid artery can be clamped once the solution leaks through these arteries
6	Store the specimen in buckets with ethanol 70% solution for at least 3 days to further fixate the tissues.

^aIf more brain tissue fixation is required, part of the solution of steps 2 and 3 can be directed through the carotid toward the head.