Table 2.
Lists of primers used for RT-qPCR analysis.
| Gene name (symbol) | Primer sequence | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
| POU class 5 homeobox 1 (OCT4) | F: AACGATCAAGCAGTGACTATTCG | 147 | 60 |
| R: AGTAGAGCGTAGTGAAGTGAGG | |||
|
| |||
| Sex determining region Y-box 2 (SOX2) | F: AGTCTCCAAGCGACGAAAAA | 189 | 60 |
| R: CCACGTTTGCAACTGTCCTA | |||
|
| |||
| Nanog homeobox (NANOG) | F: GACCGTCTCTCCTCTTCCTTCC | 157 | 60 |
| R: CGTCCTCATCTTCTGTTTCTTGC | |||
|
| |||
| BCL2-associated X protein (BAX) | F: TTTGCTTCAGGGTTTCATCC | 146 | 60 |
| R: TGTTACTGTCCAGTTCATCTCC | |||
|
| |||
| B-cell lymphoma 2 (BCL2) | F: GGGTCATGTGTGTGGAGAGC | 180 | 60 |
| R: GCCAGGAGAAGTCAAACAGAGG | |||
|
| |||
| Baculoviral IAP repeat containing 5 (BIRC5) | F: ACATTCATCTGGTTGTGCTTTCC | 157 | 60 |
| R: CACTTTCTTTGCGGTCTCTTCG | |||
|
| |||
| Peroxisome proliferator-activated receptor gamma (PPARγ) | F: GCAAGCACTTCACAAGAAACTACC | 108 | 60 |
| R: ATGGGAGTGGTCATCCATTACG | |||
|
| |||
| Fatty acid binding protein 4 (FABP4) | F: CTGGGCTCCAGAAGGTCATA | 212 | 60 |
| R: GATGATCCTGTTGGGTTTGG | |||
|
| |||
| Runt-related transcription factor 2 (Runx2) | F: TCTTCCCAAAGCCAGAGTGG | 167 | 60 |
| R: TTTAATAGCGTGCTGCCATTCG | |||
|
| |||
| Osteonectin | F: GGTGCTGAGGAAACTGAAGAGG | 180 | 60 |
| R: CTTGTTGTCGTTGCTGCATACC | |||
|
| |||
| Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | F: CCCATTCCATCTTCCAGAAA | 196 | 60 |
| R: ATCTCACGGTTCCAGTTTGC | |||