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. 2021 Jul 11;2021:5562630. doi: 10.1155/2021/5562630

Figure 1.

Figure 1

Construction of anti-CD19-CAR and anti-CD138-CAR and detection of CAR expression. (a) Diagram of the lentiviral vectors encoding third-generation CARs to target CD19 (upper) or CD138 (lower) antigen, designated as anti-CD19-CAR or anti-CD138-CAR, under the control of EF1a promoter and the restriction enzyme cutting sites EcoRI and Xbal. (b) CAR expression on the surface of CD19-HEK293T (left) or CD138-HEK293T (right) cells after transfection of anti-CD19-CAR or anti-CD138-CAR together with pCMV.dR8.2 dvpr and pCMV-VSV-G plasmids, as evaluated by flow cytometry using anti-F(ab′)2 antibody. (c) CAR expression in CD19-NK-92 (left) or CD138-NK-92 (right) cells after first round of lentiviral transduction, as similarly evaluated using anti-F(ab′)2 antibody. (d) CAR expression in CD19-HEK293T (left) or CD138-HEK293T (right) cells, as evaluated by flow cytometry based on its antigen-binding activity to His tag-rhCD19 or His tag-rhCD138.