Figure 7.

Selective cytotoxicity of dual CD19/CD138-NK-92 cells toward various hematologic cancer cells. (a) Human CML-derived K562, ALL-derived REH, BL-derived Raji, and MM-derived H929 and RPMI-8226 cells were labeled with PKH67 dye, incubated with WT-NK-92 or dual-NK-92 cells at the E : T ratio of 1 : 5 for 4 h, and stained with Annexin V-PE. Representative flow cytometric dot plots of Annexin V-positive cells (red box) in PKH67-labeled cells were shown. (b) Percentage of cancer cell death was plotted. Data are mean ± s.d. (n = 3). ^∗p < 0.05 and ^∗∗p < 0.01 versus WT-NK-92 cells; two-sided Student's t-test.