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. 2021 Jul 20;36(5):109493. doi: 10.1016/j.celrep.2021.109493

Figure 7.

Figure 7

Recoded SARS-CoV-2 mutant sCPD9s is strongly attenuated in Roborovski dwarf hamsters

(A) Weights change of mock- (n = 12) and sCPD9-infected hamsters (n = 30). Data show mean ± SD.

(B) Daily body temperature of mock- (n = 12) and sCPD9-infected hamsters (n = 30). Data show mean ± SD.

(C) Viral load in the upper (oropharyngeal swab) and lower (lung) airways and infectious virus particles detected in 50 mg of lung tissue on day 3 after infection (lung titers).

(D–O) Histopathological evaluation of the infection with sCPD9 (D–H) or SARS-CoV-2 variant (WT; I–N) on lung tissues at day 3 after infection. Left lung lobe with mild inflammatory lesions (D). Bronchioli had virtually normal columnar epithelium (E) and only occasional mild necrosuppurative bronchiolitis (F); neutrophil (black arrow). The alveolar septa showed mildly increased numbers of macrophages, and few neutrophils (G) with smaller areas of apparent pneumonia with macrophages (white arrows), neutrophils and necroses of alveolar epithelial cells (H). Normal pulmonary blood vessel (I). Typical lung of hamsters infected with WT virus (J), necrosuppurative bronchiolitis with early hyperplasia of bronchiolar epithelial cells (K), intraluminal cellular debris (hash), infiltrating neutrophils (black arrow). Bronchointerstitial pneumonia (L) with necrosis of alveolar epithelial cells, infiltration by macrophages and neutrophils (M), black arrow) and alveolar edema (M), asterisk). Endothelialitis (arrowhead) with mild to moderate perivascular edema (N, O, asterisk;). Scale bars: 1 mm (D) and (J), 50 μm (E, G, K, and L), 100 μm (I) and (N), 20 μm (insets F, H, M, and O).