REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-SARS-CoV Nucleoprotein	Novus Biological	Cat# NB100-56576; RRID:AB_838838
Anti-dsRNA, clone RJ2	EMD Millipore	Cat# MABE1134; RRID:AB_2819101
Anti-FASN	Invitrogen	Cat #MA5-31491; RRID:AB_2787122
HRP anti-rabbit secondary	Cell Signaling	Cat# 7074; RRID:AB_2099233
Anti-Rabbit AlexaFluor 488	ThermoFisher	Cat# A32731; RRID:AB_2633280
Anti-mouse AlexaFluor 647	ThermoFisher	Cat# A32728; RRID:AB_2633277
Bacterial and virus strains
SARS-CoV-2 USA/WA/1/2020	World Reference Collection for Emerging Viruses and Arboviruses – UTMB - Galveston	N/A
Chemicals, peptides, and recombinant proteins
VPS34 IN-1	Cayman Chemical	Cat# 17392
PIK-III	Cayman Chemical	Cat# 17002
Triacsin C	Cayman Chemical	Cat# 10007448
Orlistat	Cayman Chemical	Cat# 10005426
TOFA	Cayman Chemical	Cat# 10005263
C75	Cayman Chemical	Cat# 10005270
Etomoxir	Cayman Chemical	Cat# 11969
Trimetazidine	Cayman Chemical	Cat# 18165
A922500	Cayman Chemical	Cat# 10012708
Remdesivir	TargetMol	Cat# T7766
2-Bromopalmitate	Sigma-Aldrich	Cat# 21604
Microcrystalline colloidal cellulose	Sigma-Aldrich	Cat# 435244
TrueBlue HRP substrate	SeraCare	Cat# 5510-0030
Trizol	Thermo Fisher	Cat# 15596026
ezDNase	Thermo Fisher	Cat# 11766051
Superscript IV	Thermo Fisher	Cat# 18090010
AmpliTaq Gold 360	Thermo Fisher	Cat# 439881
PerFeCTa SYBR Green FastMix	VWR	Cat# 101414-272
Blocker FL Fluroescent Blocker	Thermo Fisher	Cat# 37565
Fatty Acid Free BSA	Sigma-Aldrich	Cat# A8806-5G
Palmitic Acid	Sigma-Aldrich	Cat# P0500
Oleic Acid	Sigma-Aldrich	Cat# O1008
Lipofectamine 2000	Invitrogen	Cat# 11668500
Streptococcus pyogenes NLS-Sp.Cas9-NLS (SpCas9) nuclease	Aldevron	Cat# 9212
SF Buffer	Lonza	Cat# V5SC-2002
Critical commercial assays
CellTox Green Cytotoxicity Assay	Promega	Cat# G8741
CytoView-Z 96-well electrode plates	Axion BioSystems	Cat# Z96-IMP-96B
Experimental models: Cell lines
Vero E6	ATCC	Cat# CRL-1586
Calu-3	ATCC	Cat# HTB-55
Caco-2	ATCC	Cat# HTB-37
Caco-2 Non-Targeting sgRNA Control cells	This paper	N/A
Caco-2 FASN KO cells	This paper	N/A
Oligonucleotides
FASN sgRNA 1: 5′ - ACAUACCCGCCUUCCAGCGA	This paper	N/A
FASN sgRNA 2: 5′ - UUCUGGGACAACCUCAUCGG	This paper	N/A
FASN sgRNA 3: 5′ - UGGUGAUUGCCGGCAUGUCC	This paper	N/A
FASN Forward sequencing primer: 5′ - TAGGCCACTCTGGTGCAATG	This paper	N/A
FASN Reverse sequencing primer: 5′ - GTGTCCACCTGTTCTGGGT	This paper	N/A
SARS-CoV-2 N Genomic Forward: 5′ - TAATC AGACAAGGAACTGATTA	Schneider et al., 2021	N/A
SARS-CoV-2 N Genomic Reverse: 5′ - CGAAG GTGTGACTTCCATG	Schneider et al., 2021	N/A
SARS-CoV-2 N Subgenomic Forward: 5′ - GTTTATACCTTCCCAGGTAACAAACC	Schneider et al., 2021	N/A
SARS-CoV-2 N Subgenomic Reverse: 5′ - GTAGAAATACCATCTTGGACTGAGATC	Schneider et al., 2021	N/A
SARS-CoV-2 NSP14 Forward: 5′ - TGG GGYTTTACGGGTAACCT	Schneider et al., 2021	N/A
SARS-CoV-2 NSP14 Reverse: 5′ - AACGCGCTTAACAAAGCACTC	Schneider et al., 2021	N/A
RPS11 Forward: 5′ - GCCGAGACTATCT GCACTAC	Schneider et al., 2021	N/A
RPS11 Reverse: 5′ - ATGTCCAGCCTC AGAACTTC	Schneider et al., 2021	N/A
Recombinant DNA
pEGFP-2xFYVE	Addgene	Cat# 140047