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. 2021 Jul 1;7(7):906–913. doi: 10.1038/s41477-021-00954-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — Artificially synthesized CD half coding sequence (Supplementary Table 4) and entry vectors for step2 assembly containing FokI coding sequence were amplified with primes corresponding to the template (Supplementary Table 5). The purified PCR products were mixed with 5× In-Fusion HD Cloning Enzyme Premix (TaKaRa) and incubated at 50 °C for 15 minutes.