Fig. 5.

Expression of key constituents of the mitophagy machinery are altered in PE placentae. Mitophagy-associated protein levels of PINK1, PARK2, FUNDC1, BNIP3, and BNIP3L (a); mitophagy-associated mRNA transcript levels of PINK1, PARK2, FUNDC1, BNIP3, and BNIP3L (b); autophagy-associated protein levels of SQSTM1, GABARAPL1, LC3BI, and LC3BII (c); and autophagy-associated mRNA transcript levels of SQSTM1, GABARAPL1, OPTN, and LC3A/B (d) were assessed in PE as well as control placentae. Representative immunoblots are shown and western blots were corrected for total protein loading assessed by Ponceau S staining with adjusted contrast equally applied to the whole photograph. Black boxes around the representative pictures indicate that they were cut from the same western blot. Data is presented as fold change compared to the control placentae and as mean with SEM from n = 11 (controls), n = 12 (preeclampsia). *p ≤ 0.05. PINK1, PTEN-induced kinase 1; PARK2, E3 ubiquitin-protein ligase Parkin; FUNDC1, FUN14 domain containing 1; BNIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP3L, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; SQSTM1, Sequestosome 1; GABARAPL1, GABA Type A Receptor Associated Protein Like 1; LC3B, microtubule-associated protein 1 light chain 3 beta I/II; OPTN, optineurin; and LC3A, microtubule-associated protein 1 light chain 3 alpha