Fig. 4.

A high density of tumor-infiltrating CD30⁺OX40⁺ Tregs is associated with improved OS after surgery. a–e Multiplex-IHC/IF of representative tumor section at 200X magnification. Color scheme is as follows: CD30 (green), OX40 (red), Foxp3 (white), and DAPI (blue). Single staining for CD30 (a), OX40 (b), Foxp3 (c), DAPI (d), and triple-staining for CD30, OX40, and Foxp3 (e) are shown. White arrow: CD30⁺OX40⁺ Treg cell; T: tumor island. f–k Kaplan–Meier curves of OS and DFS of CRC patients who underwent surgery. Patients were segregated into high (green curve) and low (red curve) groups based on the density of different tumor-infiltrating Tregs: f, i CD30⁺OX40⁺ Tregs, g, j CD30^–OX40⁺ Tregs, h, k CD30⁺OX40⁻ Tregs. Survival outcomes were compared between groups using univariate analysis. P value is indicated in each graph