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. 2021 Jul 19;12:4391. doi: 10.1038/s41467-021-24643-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Representative X-ray (left) and μCT (right) images of heterotopic bone in the Achilles tendon and quantitative analysis in FGFR3^f/f (n = 3) and FGFR3^Prox1 mice (n = 4) at 4 weeks after tenotomy. Scale bars, 2 mm for X-ray; 1 mm for μCT. c–e Representative H&E and SOFG images of heterotopic bone and histomorphometry analysis in FGFR3^f/f and FGFR3^Prox1 mice at 4 weeks after surgery. n = 5 per group. Scale bars, 200 μm. f–i Representative immunohistochemical (IHC) staining of Sox9 (f FGFR3^f/f = 5, FGFR3^Prox1 = 4) and Osx (h FGFR3^f/f = 5, FGFR3^Prox1 = 4) and relative quantitative analysis (g, i). Scale bars, 100 μm. j, k Representative X-ray (left) and μCT (right) images of ectopic bone in the tendon and quantitative analysis (k) in FGFR3^f/f (n = 3) and FGFR3^Prox1 mice (n = 3) at 8 weeks after Achilles tenotomy. Scale bars, 2 mm for X-ray; 1 mm for μCT. l–n Representative H&E and SOFG images of ectopic bone and histomorphometry analysis in FGFR3^f/f and FGFR3^Prox1 mice at 8 weeks after tenotomy. n = 5 per group. Scale bars, 200 μm. o–r Representative immunohistochemical staining of Runx2 (o FGFR3^f/f = 4, FGFR3^Prox1 = 5) and OC (q FGFR3^f/f = 4, FGFR3^Prox1 = 5) and relative quantitative analysis (p, r). Scale bars, 100 μm. All data represent mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 by unpaired two-tailed Student’s t-test.