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. 2021 Jul 19;7:61. doi: 10.1038/s41531-021-00204-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Comparison of gene expression profiles between eGFP⁺ (LMX1A⁺) and eGFP⁻ (LMX1A⁻) cells among vmDA precursors on D20. b The ratio of separated cells via MACS targeted for each marker (ALCAM, TPBG, CORIN, and CD47) on D20 with three biological replicates. c Flow cytometry analysis of LMX1A⁺ and FOXA2⁺ cells in unsorted, ALCAM⁺, TPBG⁺, and CD47⁺ populations on D20 with five biological replicates. *p < 0.05, one-way ANOVA with Bonferroni’s multiple-comparison tests. d Quantification of LMX1A⁺FOXA2⁺Marker⁺ triple-positive cells from the total population of differentiated cells on D20 with three independent experiments. **p < 0.01, ***p < 0.001, one-way ANOVA with Bonferroni’s multiple-comparison tests. e Representative fluorescent images for the expression of LMX1A (green), FOXA2 (magenta), and TPBG (red) in vmDA precursors on D20. DAPI (blue) was used as a counterstain. Scale bar, 25 μm.