Table 3.
Absolute and relative Apparent Permeability of metal(loid)s as measured by Caco-2 cell technique and percentage of metal(loid)s complexed.
Metal(loid) sources* | Chelating agents/Gut bacteria | Absolute App Permeability (× 10–6 cm/s)** | Relative App Permeability*** | % Complexed metal(loid)s |
---|---|---|---|---|
As—No IE | Control | 3.5600 | 100 | 10.2 |
As—with IE | Control | 2.8907 | 81.2 | 40.4 |
EDTA | 2.5739 | 72.3 | 45.2 | |
DMPS | 2.1542 | 60.5 | 48.1 | |
E. coli | 2.6166 | 73.5 | 57.6 | |
L. acidophilus | 2.2855 | 64.2 | 63.2 | |
Cd—No IE | Control | 0.8900 | 100 | 7.03 |
Cd—with IE | Control | 0.7405 | 83.2 | 22.4 |
EDTA | 0.6728 | 75.6 | 35.3 | |
DMPS | 0.5527 | 62.1 | 41.4 | |
E. coli | 0.5367 | 60.3 | 65.4 | |
L. acidophilus | 0.4931 | 55.4 | 68.7 | |
Hg—No IE | Control | 2.7500 | 100 | 8.01 |
Hg—with IE | Control | 2.2055 | 80.2 | 22.2 |
EDTA | 1.9580 | 71.2 | 26.1 | |
DMPS | 1.7078 | 62.1 | 24.3 | |
E. coli | 1.9883 | 72.3 | 34.3 | |
L. acidophilus | 1.7133 | 62.3 | 37.6 | |
Pb—No IE | Control | 0.1700 | 100 | 10.2 |
Pb—with IE | Control | 0.1399 | 82.3 | 33.2 |
EDTA | 0.1229 | 72.3 | 36.4 | |
DMPS | 0.1022 | 60.1 | 37.3 | |
E. coli | 0.1059 | 62.3 | 63.3 | |
L. acidophilus | 0.0904 | 53.2 | 65.6 |
*NO IE, No Intestinal Extract; with IE, with Intestinal Extract; **Absolute Apparent permeability is calculated from Eq. (1) (× 10–6 cm/s); ***Relative Apparent permeability is calculated from Eq. (2); absolute permeability for metal(loid) alone (No IE) is taken as 100%
Ethylene diamine tetraacetic acid (EDTA) and 2,3-dimercapto-1-propanesulfonic acid (DMPS); Escherichia coli and Lactobacillus acidophilus.