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. 2021 Jul 19;11:14733. doi: 10.1038/s41598-021-94282-6

Figure 5.

Figure 5

Temperature increase reduces coupling latency. (a) Spikes from a propagating neuron detected at E8 and E9 coupled to a postsynaptic unit in E5. At 30 °C the average latency between propagation signal spikes and the CCG peak in E5 was 2.75 ± 0.47 ms. Temperature in the same culture was increased to 32 °C and 36 °C sequentially, the average latency of the CCG peaks were 2.15 ± 1.09 ms and 1.80 ± 0.91 ms, respectively (1 MEA was used over 3 recording sessions for (a). (b) The distribution of average latency of presynaptic propagation signals coupled to postsynaptic units at 30 °C is significantly different than the average latency of the same couplings at 36 °C (1.76 ± 0.8 ms at 30 °C, 1.41 ± 0.7 ms at 36 °C; n = 23; P < 0.0001, paired t-test, two-sided). (c) Average latency of couplings at 30 °C versus the change in average latency of the same couplings at 36 °C. (7 MEAs were used with 2 recording sessions per MEA, 14 total sessions, for (b) and (c)).