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. 2021 Jul 16;7:181. doi: 10.1038/s41420-021-00571-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Macrophages collected from peritoneal lavage at 24 h after pretreated with erlotinib or 3-AB with LPS treatment. a, c Immunoblotted for PARP-1 activation, PAR formation, and GAPDH. b, d Mensuration of PARP-1 (b) and PAR (d) expressions. e Immunoblot analysis of AIF and Lamin B amount in nuclei. f Mensuration of AIF expression in nuclei. g Fluorescence images depicting AIF translocation (up panel; scale bar, 100 μm). Error bars represent SD (n = 3). *P < 0.05 as compared with NC; ^†P < 0.05 as compared with LPS group.