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. 2021 Jul 16;7:181. doi: 10.1038/s41420-021-00571-4

Fig. 4. Cell surface TLR4 expression in response to LPS could be inhibited by erlotinib.

Fig. 4

a, b Cell surface TLR4 expression in RAW264.7 cells was analyzed by flow cytometry. ce Immunoblot analysis of p-p38, t-p38, p-ERK1/2, t-ERK1/2, p-AKT, t-AKT in RAW264.7 cells treated with LPS for 3 h with or without erlotinib pretreatment for 30 min. f, h, j, l ROS production was measured with DCFH2-DA by FACS analysis. g, i, k, m Mean fluorescence intensity of ROS production. Error bars represent SD (n = 3). *P < 0.05 as compared with NC; P < 0.05 as compared with LPS group.